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total nhe3  (StressMarq)


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    Structured Review

    StressMarq total nhe3
    Total Nhe3, supplied by StressMarq, used in various techniques. Bioz Stars score: 93/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/total nhe3/product/StressMarq
    Average 93 stars, based on 43 article reviews
    total nhe3 - by Bioz Stars, 2026-02
    93/100 stars

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    Phosphorylation sites of quantified phosphopeptides that increased in abundance in response to the cAMP-generating hormone mixture ( n = 3)

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Quantitative phosphoproteomic analysis reveals cAMP/vasopressin-dependent signaling pathways in native renal thick ascending limb cells

    doi: 10.1073/pnas.1007424107

    Figure Lengend Snippet: Phosphorylation sites of quantified phosphopeptides that increased in abundance in response to the cAMP-generating hormone mixture ( n = 3)

    Article Snippet: A rabbit polyclonal antibody (H7644) against total NHE3 was generated (Lofstrand Labs) against a synthetic peptide corresponding to amino acids 621–640 of rat NHE3 (RefSeq: {"type":"entrez-protein","attrs":{"text":"NP_036786","term_id":"6981562","term_text":"NP_036786"}} NP_036786 ) and affinity purified ( SI Materials and Methods ).

    Techniques:

    Immunoblot confirmation of regulated sites. Immunoblotting with phospho-specific antibodies confirmed phosphorylation of NKCC2 at Ser126 (A, Top) and Ser874 (A, Middle), NHE3 at Ser552 (B, Top), and β-catenin at Ser552 (C, Top) in paired vehicle- and hormone-treated mTAL suspensions. In each case (A–C), the respective holo-protein was also probed. Bar graph shows results of densitometry analysis of immunoblots. H, hormone treated; V, vehicle. Error bars indicate SEM (n = 3). The asterisk indicates statistical significance (P < 0.05).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Quantitative phosphoproteomic analysis reveals cAMP/vasopressin-dependent signaling pathways in native renal thick ascending limb cells

    doi: 10.1073/pnas.1007424107

    Figure Lengend Snippet: Immunoblot confirmation of regulated sites. Immunoblotting with phospho-specific antibodies confirmed phosphorylation of NKCC2 at Ser126 (A, Top) and Ser874 (A, Middle), NHE3 at Ser552 (B, Top), and β-catenin at Ser552 (C, Top) in paired vehicle- and hormone-treated mTAL suspensions. In each case (A–C), the respective holo-protein was also probed. Bar graph shows results of densitometry analysis of immunoblots. H, hormone treated; V, vehicle. Error bars indicate SEM (n = 3). The asterisk indicates statistical significance (P < 0.05).

    Article Snippet: A rabbit polyclonal antibody (H7644) against total NHE3 was generated (Lofstrand Labs) against a synthetic peptide corresponding to amino acids 621–640 of rat NHE3 (RefSeq: {"type":"entrez-protein","attrs":{"text":"NP_036786","term_id":"6981562","term_text":"NP_036786"}} NP_036786 ) and affinity purified ( SI Materials and Methods ).

    Techniques: Western Blot